Serving Your Proteomics Needs
Biotinylation is the process of covalently attaching biotin to a protein. Due to the strong affinity between biotin and streptavidin, the biotinylated proteins have been widely used to study protein-protein interactions. Applied Biomics’ 2D Fluorescent Western Blot platform offers high sensitivity detection and accurate quantitation of Biotinylated proteins in any protein samples.
Representative PTM 2D Western Blots:
|1. Phosphorylation||6. Nitrotyrosine|
|2. Ubiquitination||7. Biotinylation|
|3. Acetylated-Lysine||8. Succinyl-Lysine|
|4. Sulfo-Tyrosine||9. Citrullination|
|5. Methyl-Arginine||10. 4-hydroxynonenal (4-HNE)|
Example: Human lung cancer cells
Proteins from Human lung cancer cells were extracted, followed by 2D DIGE Western blot using Applied Biomics’ protocols. Protein biotinalytion was revealed using CF647-labeled strepavidin (Biotium, Fremont, California).
Human lung cancer cell protein
Protein / Biotin
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