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4-HNE Protein 2D Western Blot: Detect & Quantify




Our 4-HNE Protein 2D Western Blot platform offers high sensitivity detection and accurate quantitation of 4-HNE modified Protein in any protein samples. 4-HNE, a highly toxic and most abundant stable end product of lipid peroxidation, has been implicated in the tissue damage, dysfunction and injury associated with aging and other pathological states such as cancer, Alzheimer, diabetes, cardiovascular and inflammatory complications. Further, 4-HNE, as a oxidative stress marker, also plays a key role in cell signal transduction. Applied Biomics’ 2D Fluorescent Western Blot has been successfully applied in studying the functions of 4-HNE modified Proteins.

Here are some representative applications:

1. Phosphorylation 6. Nitrotyrosine
2. Ubiquitination 7. Biotinylation
3. Acetylated-Lysine 8. Succinyl-Lysine
4. Sulfo-Tyrosine 9. Citrullination
5. Methyl-Arginine 10. 4-hydroxynonenal (4-HNE)

10. 4-Hydroxynonenal (4-HNE)

Example: Mouse liver protein

4-HNE forms protein adducts during oxidative stress. This modification adversely affects the structure and functions of many proteins, and has been hypothesized to play a key role in cell signal transductions. In the following example, proteins from drug treated Mouse liver were extracted, followed by 4-HNE Protein 2D Western Blot using Applied Biomics’ protocols.


Mouse Liver Total Protein

PTM 2D Western Blot 4-HNE

Anti-4-HNE Western Blot

PTM 2D Western Blot 4-HNE

Total Protein / Anti-4-HNE Western

PTM 2D Western Blot 4-HNE

If you are interested, please follow these steps to obtain more information and order this service.