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Identify Mono-Methylation-site




Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Identification of Mono-Methylation-site

Turnaround time is 5-7 business days. Please see the procedure of Mono-Methylation-site Identification for detailed steps of this service. Price covers the identification of Mono-Methylation-site for 1 protein per sample, following are the major steps:

1) Reduction and alkylation
2) Trypsin digestion
3) Peptide extraction
4) Desalting
5) NanoLCMSMS on peptides matching the mass of methylated peptides from virtual digest
6) Standard database search against one species in NCBI or SwissProt database using MASCOT
7) Data report:
- Top 10 hits via MASCOT search
- list of precursors with predicated methylated sites
- MSMS spectrums on precursors showing an immonium ion peak at 98 m/z

Price does NOT cover: 1) protein identification; 2) sample preparation; 3) data request in addition to the standard report.

If a customer does not provide protein sequence or accession number, an additional $150 will be charged for protein identification.