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Reproducibility

 

 

 

Reproducibility 1: in-gel 2D DIGE and across gel 2D DIGE

Reproducibility evaluation: same cell lysate sample was used for different CyDye labeling.
This experiment was also repeated, and the image data was analyzed by DeCyder software.

DeCyder software analysis

 
Cy3/Cy2
Cy3/Cy5
Cy2/Cy5
Within Gel-1
2 S.D: after filter
Total spot number
Included spot number
Excluded spot number

1.22873
2235
915
1320
1.17503
2210
1070
1140
1.22577
2210
933
1277
Within Gel-2
2 S.D: after filter
Total spot number
Included spot number
Excluded spot number

1.21323
2256
1026
1230
1.18648
2250
1344
906
1.22116
2246
1.95
1151
Across Gels
2 S.D after filter

1.29214
1.25791
1.31146

 

Reproducibility 2: Stability of lysate stored at – 80 C. Same cell lysate sample was thaw and freeze different times followed by labeling by different minimal CyDye and 2D DIGE.

Reproducibility: 2D DIGE image
Reproducibility: 2D DIGE image
Reproducibility: 2D DIGE image
Reproducibility: 2D DIGE image

 

Highly Reproducible

WT/S33A (pair 1)

Reproducibility: 2D DIGE image

WT/S33A (pair 2)

Reproducibility: 2D DIGE image

 

Two pairs of mouse liver protein expression analyzed by 2D DIGE