Home > Tech Support > Host Cell Protein (HCP) by DIGE vs. Elisa
ELISA | Standard 2D Western Blot | 2D DIGE Western Blot | |
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Two | One | |
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Silver Staining | CyDye Labeling | |
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No | ![]() |
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No | ![]() |
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No | ![]() |
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No | No | ![]() |
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No | No | ![]() |
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Low | Low | High |
Inaccurate: In-direct reaction of antibodies with the antigen’s associated proteins |
Protein spots are counted from the gel, NOT from the membrane |
Transferred protein spots are scanned directly from the membrane |
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False negative: 1) Coated Ab does not capture all HCP; 2) Surface denaturation of coating Ab can affect detection |
Error caused by: 1) Gel to gel variation; 2) Gel to membrane variation |
Accurate: Protein and Antibody signal on the same gel, thus avoiding any ambiguity |
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Lower | Lower | Higher |