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> Tech Support > Host Cell Protein (HCP) by DIGE vs. Elisa


Advantages of DIGE Western Blot Over ELISA and Standard 2D In HCP Detection

 

 

 

  ELISA Standard 2D Gel 2D DIGE


Number of gels for each Ab

  Two One


Detection Method

  Silver Staining CyDye Labeling


HCP Composition and profiling

No


Detect protein modification

No


Detect protein degradation

No


Protein and WB in same gel

No No


In-gel protein and WB comparison

No No


Accuracy

Low Low High


Inaccurate : In-direct reaction of antibodies with the antigen’s associated proteins


No QC or Detection of transferred proteins on Membrane


Protein image is scanned on the membrane


False negative: Coated Ab does not capture all HCP; Surface denaturation of coating Ab can affect detection


Error in matching spots between gel to gel, and gel to the transferred membrane


Protein and Antibody signal on the same gel, thus avoiding any ambiguity


Consistency and Reproducibility

Lower Lower Higher