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Accuracy

 

 

 

2D DIGE is accurate and highly reproducible. The following example used the same protein extract labeled separately with Cy3 and Cy5, and labeled extracts were resolved using 2D DIGE to demonstrate in-gel reproducibility. All protein spots matched exactly in the overlaid picture. This easily locates the differentially expressed protein species.

Cy3- liver lysate

Cy3- liver lysate

Cy5- liver lysate

Cy5- liver lysate

Cy3 / Cy5 -Overlay

Cy3/Cy5 -Overlay

 

2D DIGE gel 1
2D DIGE Gel 1+2
2D DIGE Gel 2
Prooteomics 2d dige gel