We offer the following services in serum 2D DIGE:

A. Improved Serum 2D DIGE without Immuno-depletion

B. Immuno-depletion of Serum Abundant Proteins

A. Improved Serum 2D DIGE Without Immuno-depletion

Applied Biomics has developed a protocol that significantly improves the performance of standard protocol. For details please click here to see
our Publication on Genetic Engineering & Technology News. Please see below for examples using ABO (Applied Biomics) improved serum
2D DIGE protocol.


* Example 1: Human Serum Sample: significantly more protein spots can be profiled by ABO protocol.

Standard 2D protocol (pH3-10)

ABO pH3-10 protocol

ABO pH4-7 protocol

* Example 2: Analysis of Preclinical Trial Mouse Serum Before and After Drug Feeding Using ABO Protocol. Four paired mouse
serum samples were collected before and after 4 different treatments, then each paired samples were analyzed by 2D DIGE using pH3-10
nonlinear IPG strip. The overlay images showed the difference between the serum samples collected before (in green color) and after (in red color) the drug feeding (18 hours). In the image overlay, the circled spots (in red) indicated a protein with increased abundance due to drug feeding. The 3-D view of the same spot is generated by DeCyder software.

B. Immuno-depletion Of Serum Abundant Proteins For 2D DIGE

The Challenge of Biomarker Discovery from Plasma/Serum

Transferrin: 76 kDa Fibrinogen: 65 kDa (a), 51 kDa (g) a1-antitrypsin: 56 kDa IgG: 150 kDa HSA: 67 kDa IgA: 180 kDa IgM: 750 kDa Transferrin: 79 kDa a2-Macroglobulin: 160 kDa Haptoglobin: 43 kDa alpha 1-acid glycoprotein: 23 kDa APO A-I, APO A-II: 23 kDa
Protein dynamic range: 10 magnitude of difference in amount

We offer the following serum abundant protein immuno-depletion services:

1) Human Serum: Albumin/IgG depletion

2) Human Serum: IgY-12 depletion (12 most abundant human plasma proteins: Human Serum Albumin, IgG, Fibrinogen, Transferrin, IgA, IgM, Haptoglobin, a2-Macroglobulin, a1-Acid Glycoprotein, a1-Antitrypsin and HDL (Apo A-I & Apo A-II).

3) Mouse Serum: Albumin/IgG depletion

4) Rat Serum: Albumin/IgG depletion

5) Monkey Serum: Albumin/IgG depletion

* Example: Human plasma with and without IgY-12 Immuno-depletion (pH4-7)

Plasma without immuno-depletion

Plasma after immuno-depletion