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Phosphorylation and Other Post-Translational Modifications (PTM) 2D Western Blot

 

 

 

1. Phospho-2D Western Blot
2. Ubiquitination
3. Acetylated-Lysine
4. Sulfo-Tyrosine
5. Methyl-Arginine
6. Nitrotyrosine

7. Biotinylation

2. Ubiquitination

Example.
Proteins were extracted from ovarian epithelial cells or mouse liver (treated after long-term feeding with 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC), using Applied Biomics 2D DIGE protocol.

A. Protein – Gel image (mouse liver)

PTM 2D Western Blot Ubiquitination
B. Protein – Gel image (ovarian cells)

PTM 2D Western Blot Ubiquitination

C. Anti-Ubiquitin Western Blot (mouse liver)

PTM 2D Western Blot Ubiquitination

D. Anti-Ubiquitin Western Blot (ovarian cells)

Anti-Ubiquitin Western Blot

E. Protein + WB overlay (mouse liver)

PTM 2D Western Blot Ubiquitination

F. Protein + WB Overlay (ovarian cells)

PTM 2D Western Blot Ubiquitination