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Ubiquitinated Protein 2D Western Blot: Detect & Quantify

 

 

 

Our Ubiquitinated Protein 2D Western Blot platform offers high sensitivity detection and accurate quantitation of Protein Ubiquitination in any protein samples. Ubiquitin is a small protein (8.6 kDa) found in most tissues of eukaryotic organisms. Ubiquitination is a process through which ubiquitin molecules are attached to protein substrates for protein degradation. Ubiquitination regulates the degradation, cellular location and activities of proteins. Applied Biomics’ 2D Fluorescent Western Blot has been successfully applied in studying the functions of Protein Ubiquitination.

Representative PTM 2D Western Blots:

1. Phosphorylation 6. Nitrotyrosine
2. Ubiquitination 7. Biotinylation
3. Acetylated-Lysine 8. Succinyl-Lysine
4. Sulfo-Tyrosine 9. Citrullination
5. Methyl-Arginine 10. 4-hydroxynonenal (4-HNE)



2. Ubiquitination

Example 1: Mouse liver

Proteins from drug treated Mouse liver were extracted, followed by 2D DIGE Western blot using Applied Biomics’ protocols.

 

Mouse liver protein

PTM 2D Western Blot Ubiquitination

Anti-Ubiquitin

PTM 2D Western Blot Ubiquitination

Protein / Anti-Ubiquitin

PTM 2D Western Blot Ubiquitination

 

Example 2: Ovarian cells

 

Ovarian cell protein

PTM 2D Western Blot Ubiquitination

Anti-Ubiquitin

PTM 2D Western Blot Ubiquitination

Protein / Anti-Ubiquitin

PTM 2D Western Blot Ubiquitination

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