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Phosphorylation and Other Post-Translational Modifications (PTM) 2D Western Blot

 

 

 

1. Phospho-2D Western Blot
2. Ubiquitination

3. Acetylated-Lysine
4. Sulfo-Tyrosine
5. Methyl-Arginine

6. Nitrotyrosine
7. Biotinylation

6. Nitrotyrosine

Example:
Proteins were extracted out from Mouse liver (treated after long-term feeding with 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC), using Applied Biomics 2D DIGE protocols.

A. Protein – Gel image (mouse cells)

PTM 2D Western Blot Nitrotyrosine
B. Anti-Nitrotyrosine WB

PTM 2D Western Blot Nitrotyrosine

C. Protein + WB overlay

PTM 2D Western Blot Nitrotyrosine