1. Phospho-2D Western Blot
2. Ubiquitination
3. Acetylated-Lysine
4. Sulfo-Tyrosine
5. Methyl-Arginine
6. Nitrotyrosine
7. Biotinylation

6. Nitrotyrosine

Example:
Proteins were extracted out from Mouse liver (treated after long-term feeding with 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC), using Applied Biomics 2D DIGE protocols.

A. Protein – Gel image (mouse cells)	B. Anti-Nitrotyrosine WB
C. Protein + WB overlay