Nitrotyrosine 2D Western Blot: Detect & Quantify

Our Nitrotyrosine 2D Western Blot platform offers high sensitivity detection and accurate quantitation of Nitrated proteins in any protein samples. Nitrotyrosine is formed by tyrosine nitration. Nitrotyrosine is a marker of cell damage, inflammation as well as NO (nitric oxide) production. Nitrotyrosine has been associated with many disease conditions such as rheumatoid arthritis, septic shock and coeliac disease. Applied Biomics’ 2D Fluorescent Western Blot has been successfully applied in studying the functions of Nitrated protein. In addition, we offer mass spectrometry service to identify the Nitrotyrosine sites.

Representative PTM 2D Western Blots:

1. Phosphorylation	6. Nitrotyrosine
2. Ubiquitination	7. Biotinylation
3. Acetylated-Lysine	8. Succinyl-Lysine
4. Sulfo-Tyrosine	9. Citrullination
5. Methyl-Arginine	10. 4-hydroxynonenal (4-HNE)

6. Nitrotyrosine

Example: Mouse liver

Proteins from drug treated Mouse liver were extracted, followed by Nitrotyrosine 2D Western Blot using Applied Biomics’ protocols.

Mouse Liver Total Protein

Anti-Nitrotyrosine Western Blot

Total Protein / Anti-Nitrotyrosine Western

If you are interested, please follow these steps to obtain more information and order this service.