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Phosphorylation and Other Post-Translational Modifications (PTM) 2D Western Blot

 

 

 

1. Phospho-2D Western Blot
2. Ubiquitination

3. Acetylated-Lysine
4. Sulfo-Tyrosine
5. Methyl-Arginine
6. Nitrotyrosine
7. Biotinylation

5. Methyl-Arginine

Example:
Proteins were extracted from Mouse mammary epithelial cells, using Applied Biomics 2D DIGE protocol.

A. Protein – Gel image (mouse cells)

PTM 2D Western Blot Methyl-Arginine
B. Anti-Methyl-Arginine WB

PTM 2D Western Blot Methyl-Arginine

C. Protein + WB overlay

PTM 2D Western Blot Methyl-Arginine