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Methylation 2D Western Blot: Detect & Quantify

 

 

 

Our Methylation Western Blot platform offers high sensitivity detection and accurate quantitation of proteins with Methyllysine or Methylarginine in any protein samples. Protein methylation such as Methyllysine or Methylarginine is formed by addition of methyl groups to Lysine or Argnine residue of proteins. Protein methylation has been most studied in histones. Methylated histones on certain residues can repress or activate gene expression. Applied Biomics’ 2D Fluorescent Western Blot has been successfully applied in studying the functions of Methylated protein. In addition, we offer mass spectrometry service to identify the methylation sites.

Representative PTM 2D Western Blots:

1. Phosphorylation 6. Nitrotyrosine
2. Ubiquitination 7. Biotinylation
3. Acetylated-Lysine 8. Succinyl-Lysine
4. Sulfo-Tyrosine 9. Citrullination
5. Methyl-Arginine 10. 4-hydroxynonenal (4-HNE)



5. Methyl-Arginine

Example 1: Mouse mammary epithelial cells

Proteins from Mouse mammary epithelial cells were extracted, followed by Methylation 2D Western Blot using Applied Biomics’ protocols.

 

Mouse Epithelial Cell Protein

PTM 2D Western Blot Methyl-Arginine

Anti-Methylarginine Western Blot

PTM 2D Western Blot Methyl-Arginine

Protein / Anti-Methylarginine Western

PTM 2D Western Blot Methyl-Arginine

 

Example 2: BHK cells

 

BHK Cell Protein

PTM 2D Western Blot Methyl-Arginine

Anti-Methylarginine Western Blot

PTM 2D Western Blot Methyl-Arginine

Protein / Anti-Methylarginine Western

PTM 2D Western Blot Methyl-Arginine

If you are interested, please follow these steps to obtain more information and order this service.