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Detection of Citrullinated Proteins by 2D Western Blot

 

 

 

Citrullination or deimination of protein is the conversion of amino acid Arginine to Citrulline in a protein. This increases the hydrophobicity of the protein, which can change the protein folding, thus affect the protein structure and functions. Becuase Citrulline is not one of the 20 standard amino acids, the immune system can attack citrullinated proteins, leading to rheumatoid arthritis and multiple sclerosis. Applied Biomics’ 2D Fluorescent Western Blot platform offers high sensitivity detection and accurate quantitation of Citrulline in any protein samples. In addition, we offer mass spectrometry service to identify the citrullination sites. This platforms has been successfully applied in studying the functions of citrullinated proteins.

Representative PTM 2D Western Blots:

1. Phosphorylation 6. Nitrotyrosine
2. Ubiquitination 7. Biotinylation
3. Acetylated-Lysine 8. Succinyl-Lysine
4. Sulfo-Tyrosine 9. Citrullination
5. Methyl-Arginine 10. 4-hydroxynonenal (4-HNE)



9. Citrullination

Example: Human T-cells

In the following example, proteins from Human T-cells were extracted, followed by 2D DIGE Western blot using Applied Biomics’ protocols.

 

Human T-cell protein

PTM 2D Western Blot Lysine Succinylation

Anti-Citrulline

PTM 2D Western Blot Lysine Succinylation

Protein / Anti-Citrulline

PTM 2D Western Blot Lysine Succinylation

If you are interested, please follow these steps to obtain more information and order this service.