Call: 1 (510) 887 0889
Email: support@appliedbiomics.com
Applied Biomics

Serving Your Proteomics Needs




Home
> Proteomics Services > Phosphorylation and Other Post-Translational Modifications (PTM) 2D Western Blot


Phosphorylation and Other Post-Translational Modifications (PTM) 2D Western Blot

 

 

 

1. Phospho-2D Western Blot
2. Ubiquitination

3. Acetylated-Lysine
4. Sulfo-Tyrosine
5. Methyl-Arginine
6. Nitrotyrosine

7. Biotinylation

7. Biotinylation

Example
Proteins were extracted from human lung cancer cells. 2D DIGE gel was run on using pH 3-10 linear IPG strip and then transferred to the PVDF membrane.
A: Membrane 2D image was scanned after transfer
B: Protein biotinalytion (2) was revealed using CF647-labeled strepavidin (Biotium, Hayward, California)
C: Image overlay using ImageQuant software

A. Protein – Membrane image

PTM 2D Western Blot Biotinylation
B. Biotin blotting

PTM 2D Western Blot Biotinylation

C. Protein + Biotin blotting overlay

PTM 2D Western Blot Biotinylation