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Biotinylated Protein 2D Western Blot: Detect & Quantify

 

 

 

Our Biotinylated Protein 2D Western Blot platform offers high sensitivity detection and accurate quantitation of Protein Biotinylation in any protein samples. Biotinylation is the process of covalently attaching biotin to a protein. Due to the strong affinity between biotin and streptavidin, the biotinylated proteins have been widely used to study protein-protein interactions.

Representative PTM 2D Western Blots:

1. Phosphorylation 6. Nitrotyrosine
2. Ubiquitination 7. Biotinylation
3. Acetylated-Lysine 8. Succinyl-Lysine
4. Sulfo-Tyrosine 9. Citrullination
5. Methyl-Arginine 10. 4-hydroxynonenal (4-HNE)



7. Biotinylation

Example: Human lung cancer cells

Proteins from Human lung cancer cells were extracted, followed by Biotinylated Protein 2D Western Blot using Applied Biomics’ protocols. Protein biotinalytion was revealed using CF647-labeled strepavidin (Biotium, Fremont, California).

 

Human Lung Cancer Cell Protein

PTM 2D Western Blot Biotinylation

Anti-Biotin Western Blot

PTM 2D Western Blot Biotinylation

Protein / Anti-Biotin Western Blot

PTM 2D Western Blot Biotinylation

If you are interested, please follow these steps to obtain more information and order this service.