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Phosphorylation and Other Post-Translational Modifications (PTM) 2D Western Blot

 

 

 

1. Phospho-2D Western Blot
2. Ubiquitination
3. Acetylated-Lysine
4. Sulfo-Tyrosine
5. Methyl-Arginine
6. Nitrotyrosine
7. Biotinylation

3. Acetylated-Lysine

Example:
Proteins were extracted from mouse liver (treated after long-term feeding with 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC), or from rat liver, using Applied Biomics 2D DIGE protocol.

A. Protein – Gel image (mouse liver)

PTM 2D Western Blot Acetylated-Lysine
B. Protein – Gel image (Rat liver)

PTM 2D Western Blot Acetylated-Lysine

C. Anti-Acetylated-Lysine WB (mouse liver)

PTM 2D Western Blot Acetylated-Lysine

D. Anti-Acetylated-Lysine WB (Rat liver)

PTM 2D Western Blot Acetylated-Lysine

E. Protein + WB overlay (mouse liver)

PTM 2D Western Blot Acetylated-Lysine

F. Protein + WB Overlay (rat liver)

PTM 2D Western Blot Acetylated-Lysine