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Detection of 4-Hydroxynonenal (4-HNE) Proteins by 2D Western Blot




4-Hydroxy 2-NonEnal (4-HNE), a highly toxic and most abundant stable end product of lipid peroxidation, has been implicated in the tissue damage, dysfunction and injury associated with aging and other pathological states such as cancer, Alzheimer, diabetes, cardiovascular and inflammatory complications. Further, 4-HNE, as a oxidative stress marker, also plays a key role in cell signal transduction. Applied Biomics’ 2D Fluorescent Western Blot platform offers high sensitivity detection and accurate quantitation of 4-HNE in any protein samples.

Here are some representative applications:

1. Phosphorylation 6. Nitrotyrosine
2. Ubiquitination 7. Biotinylation
3. Acetylated-Lysine 8. Succinyl-Lysine
4. Sulfo-Tyrosine 9. Citrullination
5. Methyl-Arginine 10. 4-hydroxynonenal (4-HNE)

10. 4-Hydroxynonenal (4-HNE)

Example: Mouse liver protein

4-HNE forms protein adducts during oxidative stress. This modification adversely affects the structure and functions of many proteins, and has been hypothesized to play a key role in cell signal transductions. In the following example, proteins from drug treated Mouse liver were extracted, followed by 2D DIGE Western blot using Applied Biomics’ protocols.


Mouse liver protein

PTM 2D Western Blot 4-HNE


PTM 2D Western Blot 4-HNE

Protein / Anti-4-HNE

PTM 2D Western Blot 4-HNE

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