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Identify Phosphorylation Site

 

 

 

Each experiment covers identifying ALL Phosphorylation sites (Ser, Thr and Tyr) in a single protein per sample. This service works the best with purified proteins or IP samples with target proteins enriched. Customers can send samples in-gel or in-solution, and provide the amino acid sequence or accession number of the target protein.

Identification of Phosphorylation-site involves enriching phosphorylated peptides followed by mass spectrometry. The enrichment is critical since phosphorylated proteins often represent 1-2% of the total protein population. In each run, a positive control consisting of a peptide with a known phosphosite is subjected to the same experimental conditions as the test samples. The presence of phosphosites is confirmed at by the precense of a MSMS peak showing the loss of phosphate.


Gel Bands/Spots or In-Solution Samples

Gel bands/spots 
Reduction/Alkylation & Trypsin Digestion

Reduction/Alkylation & Trypsin Digestion
Phospho-Peptide Enrichment

Phosphorylated peptide enrichment
MS
Identification of Phosphorylated peptide site
MS/MS: Phosphorylation Site
Identification of Phosphorylation Site
Database Search for Phosphorylation Sites

If you are interested, please follow these steps to obtain more information and order this service.