Home > Proteomics Services > Identify Phosphorylation Site
Each experiment covers identifying ALL Phosphorylation sites (Ser, Thr and Tyr) in a single protein per sample. This service works the best with purified proteins or IP samples with target proteins enriched. Customers can send samples in-gel or in-solution, and provide the amino acid sequence or accession number of the target protein.
Identification of Phosphorylation-site involves enriching phosphorylated peptides followed by mass spectrometry. The enrichment is critical since phosphorylated proteins often represent 1-2% of the total protein population. In each run, a positive control consisting of a peptide with a known phosphosite is subjected to the same experimental conditions as the test samples. The presence of phosphosites is confirmed at by the precense of a MSMS peak showing the loss of phosphate.
Gel Bands/Spots or In-Solution Samples ![]() |
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Reduction/Alkylation & Trypsin Digestion ![]() |
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Phospho-Peptide Enrichment ![]() |
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MS ![]() |
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MS/MS: Phosphorylation Site ![]() |
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Database Search for Phosphorylation Sites |
If you are interested, please follow these steps to obtain more information and order this service.