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In-Gel Data Analysis

 

 

 

The data analysis of a typical 2D DIGE experiment consists of two main parts:

A. ImageQuant analysis: Gel images of individual samples and overlay of two sample images will be generated. In the overlay image, spots with green, red and yellow color indicate decreased, increased and similar expression, respectively.

WT

2D DIGE In-Gel Data Analysis

Mutant

2D DIGE In-Gel Data Analysis

Overlay: WT / Mutant

2D DIGE In-Gel Data Analysis

 

B. DeCyder DIA Analysis: Quantitative analysis and comparison of all spots between different samples. The differential protein expression is assessed by Differential In-gel Analysis (DIA), giving the high accuracy and reproducibility. The data will provide: 1) General statistic data (2 S.D. value, total spot number detected, number of decreased and increased spots etc.); 2) protein expression ratio (fold of change); 2) 3-D image view of the corresponding spot.

Upper left: Gel spot view
(left: control, right: treated).
Red: increased;
Blue: decreased
  Upper right: Spot distribution.
threshold level, the number of spots
that are increased, decreased and within the threshold
  2D DIGE In-Gel Data Analysis Threshold
2 SDvalue



The height of a spot indicates the volume of the spot, the curves indicate the number of spots

 

 

 


Selected spot



 

Lower left: 3D view.
Characteristics of the selected spot
  Lower right: Table view.
All spots of interest. The fold change
(increase or decrease) of each spot is indicated. The spot that shows at the 3D view is highlighted.

 

 

Examples: In-gel Image Analysis Using DeCyder Software

 

2D DIGE In-Gel Data Analysis