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> Proteomics Services > HCP Test: HCP Contaminant

HCP Analysis: Monitor Product Purity & HCP Contaminant

HCP Analysis: Antibody Coverage
HCP Analysis: Detect And Quantitate 3 HCP Samples In 1 Test


Monitoring product purity / HCP Contaminant is another important application of HCP assay. Due to the different spot patterns from samples in the purification process, External Protein Markers (EPMs) can be added to the sample and used to adjust the protein quantitation. Applied Biomics has developed several EPMs at different pH and Mw for you to choose from.

The EPMs can serve as a standard to accurately assess product purity in the following areas:

Evaluating of the purity of therapeutic antibodies, recombinant proteins and vaccines
 Monitoring and optimizing of bioprocess
  Characterizing batch to batch cell lines
Evaluating storage conditions of biological samples

Click to view a complete list of Applied Biomics HCP assays

Example-1: Adjust protein ratios by Applied Biomics EPMs
Example-2: Monitor product purity by Applied Biomics EPMs

Applied Biomics External Protein Markers (EPMs)


Cy3 - EPMs


Cy5 - EPMs

Overlay image

Cy3 Cy5 EPMs image overly

* EPM_1: 76 kDa, pI=6.0, 6.2, 6.6
* EPM_2: 30 kDa, pI=5.4 – 6.0
* EPM_3: 14 kDa, pI=6.8 - 7.2


Locations of Applied Biomics EPMs Relative to HCPs



Cell Lysates

Cell Lysate

Green: EPMS
Red: Cell Lysates

EMPS Cell Lysates image overlay

Example-1: Adjust Protein Ratios Using Applied Biomics EPMs

NO EPM Adjustment

protein ratios without EPM adjustment

With EPM Adjustment

Protein ratios with EPM adjustment

Spots Ratios Without EPM Adjustment Ratios With EPM Adjustment
1 1.6 3.9
2 -6.3 -2.6
3 -18.6 -7.6
4 9.2 22.5
5 6.4 15.7
6 -11.8 -4.8
7 -2.2 1.1
EPM -2.4 1.0

Example-2: Monitor Product Purity Using Applied Biomics EPMs

DeCyder Image

Purification Process

monitor product purity using Applied Biomics EPMs

1) Use DIGE Western blot information to locate all product protein (and their isoforms) - blue spots

2) Calculate % purity of Product

3) Adjust the product ratios between different purification steps using EPMs - green spots

If you are interested, please follow these steps to obtain more information and order this service.