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Host Cell Protein (HCP) Assays

 

 

 

Applied Biomics offers both Gel-based HCP Assays and Liquid-based HCP Assays that will suit your different project goals:

Platform
Gel-Based Host Cell Protein (HCP) Analysis Liquid-Based
HCP Analysis
1D DIGE 1D DIGE WB 2D Gel 2D WB 2D DIGE 2D DIGE WB Nano-LCMSMS
Optional Combined with Protein ID by MALDI-TOF/TOF or NanoLCMSMS N/A


Separate HCP by

MW MW MW and pI MW and pI MW and pI MW and pI Hydrophobicity
(& MW - optional)


Detection

CyDye label CyDye label Staining Staining CyDye label CyDye label MSMS


Sensitivity

0.2 ng 0.2 ng 1 ng 1 ng 0.2 ng 0.2 ng 10 amol


Visualize protein image

No


Protein and Antibody Signal on the same gel

No No No


Advantages






1) Quick comparison of HCPs in one dimension





1) Detailed comparison of HCPs in two dimensions
2) Accurate quantitation




1) Detailed comparison of HCPs in two dimensions
2) Accurate quantitation
3) High sensitivity
4) High consistency
5) High dynamic range


1) Detailed HCP components