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Identification of Acetylation Site

 

 

 

This service works best with purified acetylated proteins, samples can be sent to us in-gel or in solution. In addition, customer will provide the amino acid sequence or accession number of the target protein.

Identification of Acetylated-lysine sites on a single protein involves virtual digestion of the protein sequence to identify potential peptides containing acetylated lysines, followed by MS matching to the virtual digest results and MS/MS fragmentation on matching MS precursors. The presence of an acetylated lysine residue is confirmed by the identification of an immonium ion peak at 126 m/z during MS/MS.

Gel Bands/Spots or Liquid samples

Gel bands/spots

Reduction/Alkylation & Trypsin Digestion

Reduction/Alkylation & Trypsin Digestion



MS
Identification of Acetylation Site Identification of Acetylation Site



MS/MS
Identification of Acetylation Site Identification of Acetylation Site


Database Search for Acetylated Site Modification