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2D DIGE & Western Blot
2D Phospho-Western Blot

 

 

 

Two samples from Mouse liver, Control and Treated, representing before and after long-term feeding with 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC), were processed using Applied Biomics protocols.

First, samples were run on 2D DIGE (pH4-7) and the gel images were scanned using Typhoon 9400 (A, B and C).
Second, proteins from 2D gel were transferred to the membrane and membrane images were scanned (D and E).
Third, Western Blot was performed using rabbit anti-mouse keratin 8 and keratin 18 as primary antibodies followed by using CF555-labeled goat anti-rabbit Ig (Biotium, Hayward, California) as a secondary antibody, and the Western Blot images were scanned (F)

Gel Images

A. DIGE Gel image: Control sample

2D DIGE & Western Blot

 
Membrane Images

D. Membrane image (Control): scanned after transfer

2D DIGE & Western Blot

B. DIGE Gel image: Treated sample

2D DIGE & Western Blot

E. Membrane image (Treated): scanned after transfer

2D DIGE & Western Blot

C. DIGE Gel Image Overlay
Green: Control Red: Treated

2D DIGE & Western Blot

F. K8 and K18 revealed by Western blot

2D DIGE & Western Blot