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Two samples from Mouse liver, Control and
Treated, representing before and after long-term
were processed using Applied Biomics protocols.
First, samples were run on 2D DIGE (pH4-7) and the gel images were scanned using Typhoon 9400 (A, B and C).
Second, proteins from 2D gel were transferred to the membrane and membrane images were scanned (D and E).
Third, Western Blot was performed using rabbit anti-mouse keratin 8 and keratin 18 as primary antibodies followed by using CF555-labeled goat anti-rabbit Ig (Biotium, Hayward, California) as a secondary antibody, and the Western Blot images were scanned (F)
A. DIGE Gel image: Control sample
D. Membrane image (Control): scanned after transfer
|B. DIGE Gel image: Treated sample
||E. Membrane image (Treated):
scanned after transfer
|C. DIGE Gel Image Overlay
Green: Control Red: Treated
F. K8 and K18 revealed by Western blot