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Here we present a 2D DIGE gel with TWO samples, followed by Western Blot (WB). This SINGLE gel allows comparisons of protein profiling between the Control and Treated sample by 2D DIGE AND antibody detection by WB. Such analysis is only feasible using 2D DIGE Western Blot.
Step 1: Proteins were extracted from the Control and Treated Mouse liver samples, followed by 2D DIGE (pH4-7) using Applied Biomics’ protocols. The data was processed by in-gel analysis.
DIGE Gel image: Control |
DIGE Gel image: Treated |
Control / Treated |
Membrane Image: Control |
Membrane Image: Treated |
Western Blot Image: K8 and K18 Spots |