1D Western Blot

Our quantitative Western blot, with Fluorescent labeled protein samples, offers the following unique features:

1) High sensitivity: 1ng /band
2) Quantitation: Accurate quantitation of band intensity, % band, protein quantity by ImageQuantTM TL (GE Healthcare, version 7.0)
3) Multiplex Western blot: Single and Two-color Western blots are available to detect one or two antibody in a single blot

Example: 2-Color Western blot detecting time-dependent phosphorylation of 14-3-3

Total proteins were extracted from the drug treated HT29 cells under different time points. In each lane, equal amount of protein was labeled and loaded. The gel was then transferred to a PVDF membrane followed by a two-color Western blot:
A: Total proteins
B: Western blot with Mouse Anti-14-3-3 antibody
C: Western blot with Rabbit Anti-phospho14-3-3 antibody

A. HT29 Cell Total Protein		B. Mouse Anti-14-3-3 Western Blot	A+B. Image overlay
		C. Rabbit Anti-phospho14-3-3 Western	A+C. Image overlay
		B+C. 2-Color-Western Blot Overlay	A+B+C. Triple-color Overlay

If you are interested, please follow these steps to obtain more information and order this service.