Call: 1 (510) 887 0889
Email: support@appliedbiomics.com
Applied Biomics

Serving Your Proteomics Needs




Home
> Proteomics Services > Quantitative 1D Gel and 1D Western Blot


Quantitative 1D Gel and 1D Western Blot

 

 

 

We offer quantitative 1D Gel and 1D Western blot featuring:

1) High sensitivity: 1ng /band
2) Quantitative: accurate quantitation by ImageQuantTM TL (GE Healthcare, version 7.0)
3) Data output: band intensity, % band, protein quantity
4) Western blot: single, double or triple-color Western blots are available to detect one, two or three antigens in the same time

Example: Time-dependent protein phosphorylation detected by double-color 1D Western blot

A. Total Protein

Quantitative 1D Gel and 1D Western Blot







B. WB Mouse anti-14-3-3

Quantitative 1D Gel and 1D Western Blot
A+B. Image overlay

Quantitative 1D Gel and 1D Western Blot

C. WB Rabbit anti-phospho14-3-3

Quantitative 1D Gel and 1D Western Blot

A+C. Image overlay

Quantitative 1D Gel and 1D Western Blot
 
B+C. Double-WB Overlay

Quantitative 1D Gel and 1D Western Blot

A+B+C. Triple-color Overlay

Quantitative 1D Gel and 1D Western Blot


Total proteins were extracted from the drug treated HT 29 cells under different time points. In each lane, equal amount of protein was labeled and loaded. The gel was then transferred to a PVDF membrane for a two-color Western blot:
A: Total proteins
B: Western blot with mouse anti-14-3-3
C: Western blot with rabbit anti-phospho14-3-3 antibodies