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1D Western Blot

2D Western Blot

 

 

 

 

Our quantitative Western blot, with Fluorescent labeled protein samples, offers the following unique features:

1) High sensitivity: 1ng /band
2) Quantitation: Accurate quantitation of band intensity, % band, protein quantity by ImageQuantTM TL (GE Healthcare, version 7.0)
3) Multiplex Western blot: Single and Two-color Western blots are available to detect one or two antibody in a single blot

Example: 2-Color Western blot detecting time-dependent phosphorylation of 14-3-3

Total proteins were extracted from the drug treated HT29 cells under different time points. In each lane, equal amount of protein was labeled and loaded. The gel was then transferred to a PVDF membrane followed by a two-color Western blot:
A: Total proteins
B: Western blot with Mouse Anti-14-3-3 antibody
C: Western blot with Rabbit Anti-phospho14-3-3 antibody

 

A. HT29 Cell Total Protein

Quantitative 1D Gel and 1D Western Blot







B. Mouse Anti-14-3-3 Western Blot

Quantitative 1D Gel and 1D Western Blot
A+B. Image overlay

Quantitative 1D Gel and 1D Western Blot

C. Rabbit Anti-phospho14-3-3 Western

Quantitative 1D Gel and 1D Western Blot

A+C. Image overlay

Quantitative 1D Gel and 1D Western Blot
 
B+C. 2-Color-Western Blot Overlay

Quantitative 1D Gel and 1D Western Blot

A+B+C. Triple-color Overlay

Quantitative 1D Gel and 1D Western Blot

If you are interested, please follow these steps to obtain more information and order this service.