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Protein Identification

 

 

 

Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
Protein ID by MALDI-TOF/TOF for DIGE Customers 1
104A
$115/sample
104N
$135/sample
Protein ID by MALDI-TOF/TOF for External Customers
(10 or fewer samples) 1
305A
$150/sample
305N
$180/sample
Protein ID by MALDI-TOF/TOF for External Customers
(more than 10 samples) 1
305A
$135/sample
305N
$160/sample
LC-MS/MS on Low Complexity Samples
(1D Gel band, 2-5 samples) 2
331A
$450/sample
331N
$540/sample
LC-MS/MS on Low Complexity Samples
(1D Gel band, 6 or more samples) 2
331A
$380/sample
331N
$420/sample
LC-MS/MS on Moderate Complexity Samples 2
332A
call for price
332N
call for price
LC-MS/MS on Moderate to High Complexity Samples 2
334A
call for price
334N
call for price
LC-MS/MS on High Complexity Samples 2
333A
call for price
333N
call for price


1. Turnaround time is 2-5 business days. Please see procedure of protein ID for detailed steps of this service. Price covers the following:
1) Gel treatment
2) In-gel trypsin digestion
3) Peptide extraction
4) Desalting
5) Spotting
6) MALDI-TOF
7) MALDI-TOF/TOF
8) Standard database search against one species in NCBI or SwissProt database using MASCOT
9) Data report (see an example)

2. Each order should have a minimum of 2 samples. Turnaround time is 5-7 business days for 3 or more samples, and up to 10 business days for 2 samples. Please see procedure of LC-MS/MS for detailed steps of this service.

Sample types:
1) Low complexity samples: 1D gel band - Each sample should contain only one gel piece with one protein band, no bigger than 1x1x5mm in size, <10 proteins. Samples with more than one gel piece, and/or more than one protein band will be considered as moderate to high complex samples. We recommend you cut out only the area with your protein of interest (1x1x2mm size) and follow these guidelines to prepare samples
2) Moderate complexity samples: protein mixture such as column purified sample, <100 proteins 
3) Moderate to High complexity samples: Protein mixtures such as subcellular organelles, IP samples with 100 - 500 proteins
4) High complexity samples: whole cell lysate, tissue, and others with more than 500 protein mix

Price covers the following:
1) Nano-LC coupled with auto-spotting
2) MS/MS
3) Standard database search against one species in NCBI or SwissProt database using MASCOT
4) Data report (see an example)

Price does NOT cover: 1) sample preparation; 2) data request in addition to the standard report.