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2D DIGE Preparative Gel and Protein ID By Mass Spectrometry

 

 

 

Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
2D DIGE Preparative Gel (1 CyDye labeling)* 1
401A
$800
401N
$960
2D DIGE Preparative Gel (2 CyDye labeling)* 1
102A2
$1200
102N2
$1500
2D DIGE Preparative Gel (3 CyDye labeling)* 1
102A3
$1295
102N3
$1595
Spot Picking per Gel* 2
103
$250 per 96 spots
103
$250 per 96 spots
Protein ID by Mass Spectrometry (MALDI-TOF/TOF) 3
104A
$115 per spot
104N
$135 per spot
Pathways Analysis After Protein ID 4
203A
call for a quote
203N
call for a quote

* Price applies for customers who perform protein identification at Applied Biomics

1. Price covers:
1) Experimental design
2) Sample preparation and protein concentration determination
3) Fluorescence dye labeling using CyDye (1 sample for 1 CyDye labeling; 2 samples for 2 CyDye labeling; 3 samples for 3 CyDye labeling)
4) IEF and SDS-PAGE
5) Gel image scan using Typhoon scanner
6) Spot picking design and Data analysis up to 3 hours

2. Price covers:
1) picking design and set up
2) pick spots
3) free storage at -80C for 6 months.

3. Prices are discounted and only apply for customers doing 2D DIGE service with Applied Biomics. Turnaround time starting from Preparative gel to sending protein ID report is 7-10 business days. We offer high sensitivity protein identification from gel spots or bands using the latest technologies in mass spectrometry. In each run, four sensitivity standards in the amount of 1-10 femtomole are included. Our sensitivity is the range of 1-2 femtomole. Please see procedure of protein ID for detailed steps of this service. Price covers the following:
1) Gel treatment
2) In-gel trypsin digestion
3) Peptide extraction
4) Desalting
5) Spotting
6) MALDI-TOF
7) MALDI-TOF/TOF
8) Database search against NCBI database using MASCOT
9) Data report (see an example)

4. Pathway Analysis Service is provided only for customers doing the 2D DIGE and Protein ID services with Applied Biomics. The report will include the major pathway list with the following information:
1) Functional groups
2) Genes in each functional group
3) Enrichment score
4) Statistic p-value and FDR