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Serum 2D DIGE

 

 

 

We offer the following services in serum 2D DIGE:

A. Improved Serum 2D DIGE without Immuno-depletion

B. Immuno-depletion of Serum Abundant Proteins

A. Improved Serum 2D DIGE Without Immuno-depletion

Applied Biomics has developed a protocol that significantly improves the performance of standard protocol. For details please click here to see
our Publication on Genetic Engineering & Technology News. Please see below for examples using ABO (Applied Biomics) improved serum
2D DIGE protocol.


* Example 1: Human Serum Sample: significantly more protein spots can be profiled by ABO protocol.

Standard 2D protocol (pH3-10)

Serum 2D DIGE
ABO pH3-10 protocol

 Serum 2D DIGE
ABO pH4-7 protocol

 Serum 2D DIGE

 

* Example 2: Analysis of Preclinical Trial Mouse Serum Before and After Drug Feeding Using ABO Protocol. Four paired mouse
serum samples were collected before and after 4 different treatments, then each paired samples were analyzed by 2D DIGE using pH3-10
nonlinear IPG strip. The overlay images showed the difference between the serum samples collected before (in green color) and after (in red color) the drug feeding (18 hours). In the image overlay, the circled spots (in red) indicated a protein with increased abundance due to drug feeding. The 3-D view of the same spot is generated by DeCyder software.

Serum 2D DIGE

B. Immuno-depletion Of Serum Abundant Proteins For 2D DIGE

The Challenge of Biomarker Discovery from Plasma/Serum

Transferrin: 76 kDa
Fibrinogen: 65 kDa (a), 51 kDa (g)
a1-antitrypsin: 56 kDa
IgG: 150 kDa
HSA: 67 kDa
IgA: 180 kDa
IgM: 750 kDa
Transferrin: 79 kDa
a2-Macroglobulin: 160 kDa
Haptoglobin: 43 kDa
alpha 1-acid glycoprotein: 23 kDa
APO A-I, APO A-II: 23 kDa

 


 The Challenge of Biomarker Discovery from Plasma/Serum
Protein dynamic range: 10 magnitude of difference in amount  

 

We offer the following serum abundant protein immuno-depletion services:

1) Human Serum: Albumin/IgG depletion

2) Human Serum: IgY-12 depletion (12 most abundant human plasma proteins: Human Serum Albumin, IgG, Fibrinogen, Transferrin, IgA, IgM, Haptoglobin, a2-Macroglobulin, a1-Acid Glycoprotein, a1-Antitrypsin and HDL (Apo A-I & Apo A-II).

3) Mouse Serum: Albumin/IgG depletion

4) Rat Serum: Albumin/IgG depletion

5) Monkey Serum: Albumin/IgG depletion

* Example: Human plasma with and without IgY-12 Immuno-depletion (pH4-7)

Plasma without immuno-depletion

Plasma without immuno-depletion
Plasma after immuno-depletion

 plasma after immuno-depletion