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2D DIGE Phosphorylated Protein Profiling

 

 

 

Phospho-protein Staining

2D DIGE Phosphorylated Protein Profiling
Cy5 Total Protein Labeling

2D DIGE Phosphorylated Protein Profiling
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Phosphorylated Protein Profiling

Phospho-protein Staining

2D DIGE Phosphorylated Protein Profiling

Cy3 Total Protein Labeling

2D DIGE Phosphorylated Protein Profiling

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2D DIGE Phosphorylated Protein Profiling

2D DIGE Phosphorylated Protein Detection

2-D DIGE analysis of protein differential expression after the drug treatment followed by phospho-protein detection. 1. phospho-protein staining; 2. control; 3. drug treated.

Time-dependent phosphorylation changes of aconitate hydrase

 

ES
Day 2
Day 4
Day6
Day8
Day10
Time-dependent phosphorylation changes of aconitate hydrase
Time-dependent phosphorylation changes of aconitate hydrase
Time-dependent phosphorylation changes of aconitate hydrase
Time-dependent phosphorylation changes of aconitate hydrase
Time-dependent phosphorylation changes of aconitate hydrase
Time-dependent phosphorylation changes of aconitate hydrase

 

Day 2 / ES
Time-dependent phosphorylation changes of aconitate hydrase
Day 4 / ES
Time-dependent phosphorylation changes of aconitate hydrase
Day 6 / ES
Time-dependent phosphorylation changes of aconitate hydrase
Day 8 / ES
Time-dependent phosphorylation changes of aconitate hydrase
Day 10 / ES
Time-dependent phosphorylation changes of aconitate hydrase
Day 2 / ES
Time-dependent phosphorylation changes of aconitate hydrase
Day 4 / Day 2
Time-dependent phosphorylation changes of aconitate hydrase
Day 6 / Day 4
Time-dependent phosphorylation changes of aconitate hydrase
Day 8 / Day 6
Time-dependent phosphorylation changes of aconitate hydrase
Day 10 / ES
Time-dependent phosphorylation changes of aconitate hydrase

 

ES
D2 / ES
D4 / D2
D6 / D4
D8 / D6
D10 / D8
Spot # 20: phospho-Aconitate hydrase
- 1.02
- 1.05
+ 3.28
- 1.81
- 1.11
Spot # 21: phospho-Aconitate hydrase
- 1.41
+ 1.41
+ 3.54
- 1.96
-1.06
Spot # 26: phospho-Aconitate hydrase
- 1.45
+ 1.91
+ 1.50
- 1.09
- 1.03
Spot # 28: non-phospho-Aconitate hydrase
- 1.45
+1.91
+ 1.28
- 1.07
- 1.03