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2D DIGE Phosphorylated Protein Profiling
Phospho-protein Staining |
Cy5 Total Protein Labeling |
Image Overlay |
Phospho-protein Staining  |
Cy3 Total Protein Labeling  |
Image Overlay  |
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2-D DIGE analysis of protein differential expression after the drug treatment followed by phospho-protein detection. 1. phospho-protein staining; 2. control; 3. drug treated. |
Time-dependent phosphorylation changes of aconitate hydrase
|
ES
 |
Day 2
|
Day 4
|
Day6
|
Day8
|
Day10
|
 |
 |
 |
 |
 |
 |
|
Day 2 /
ES
 |
Day 4 /
ES
 |
Day 6 /
ES
 |
Day 8 /
ES
 |
Day 10
/ ES
|
|
Day 2 /
ES
 |
Day 4 /
Day 2
 |
Day 6 /
Day 4
 |
Day 8 /
Day 6
|
Day 10
/ ES
|
|
ES
|
D2 / ES
|
D4 / D2
|
D6 / D4
|
D8 / D6
|
D10 / D8
|
|
Spot # 20:
phospho-Aconitate hydrase
|
- 1.02
|
- 1.05
|
+ 3.28
|
- 1.81
|
- 1.11
|
|
Spot # 21:
phospho-Aconitate hydrase
|
- 1.41
|
+ 1.41
|
+ 3.54
|
- 1.96
|
-1.06
|
|
Spot # 26:
phospho-Aconitate hydrase
|
- 1.45
|
+ 1.91
|
+ 1.50
|
- 1.09
|
- 1.03
|
| Spot # 28: non-phospho-Aconitate hydrase |
- 1.45
|
+1.91
|
+ 1.28
|
- 1.07
|
- 1.03
|