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High Resolution Membrane Protein Proteomics Analysis

 

 

 

Membrane proteins generally have higher pI and contain post translational modifications (PTMs). It is hard to extract membrane proteins due to their hydrophobicity. As a result, membrane proteins showed poor resolution on 2D gels, presenting a challenge in proteomics studies.

Applied Biomics developed a unique method to efficiently extract membrane proteins from the prepared membrane fractions. In combination with our optimized 2D electrophoresis conditions, we offer our customers high resolution membrane proteome array to facilitate their membrane proteomics projects.

This images below showed 2D DIGE analysis of the HT29 membrane proteins of S- and G2/M-phase. The membrane complex fractions were prepared using sucrose density gradient. The membrane proteins were extracted from membrane fractions using Applied Biomics’ membrane protein extraction buffer. The 2D DIGE images revealed high resolution of membrane protein spots and clearly showed some significantly changed membrane proteins between S-and G2/M-phase.

 

S-Phase Membrane Protein

Membrane protein complex Cy3

G2/M-Phase Membrane Protein

Membrane protein complex Cy5

S-Phase / G2/M-Phase

Membrane protein complex overlay

 

S-Phase Membrane Protein

Memberane protein complex Cy3 by 2D DIGE

G2/M-Phase Membrane Protein

Membrane protein complex Cy5 by 2D DIGE

S-Phase / G2/M-Phase

Membrane protein complex overlay


If you are interested, please follow these steps to obtain more information and order this service.